should we perform semen analysis, dna fragmentation, and hypo-osmotic swelling tests together?
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abstract
semen analysis, sperm dna fragmentation (sdf) and hypo-osmotic swelling test (host) are usually performed for the evaluation of sperm fertilizing ability. there are some debates over the necessity of sdf and host incorporation in male infertility work-up.semen of 77 men was evaluated by sdf and host through three semen analyses. sperm parameters were arranged into different categories: < 5%, 5-15%, >15% for normal morphology; < 50%, 50-70%, >70 % for motility; and < 10, 10-20, 21-34, 35-50, >50 million/ml for concentration. sdf analysis was performed and values under 30% were assumed to be normal. normal range of host was considered to be >60%.only normal sperm morphology had significant relationship with df rate (p< 0.001). normal morphology, motility, and concentration of sperms had significant relationship with host (p< 0.001, 0.05, and < 0.003,respectively). there was a significant negative correlation between sperm morphology and df rate. the correlations between sperm parameters and percentage of host were significantly positive (r: 0.44, 0.19, and 0.32 for morphology, motility, and concentration, respectively). receiver operating characteristic curve (roc) showed that sperm morphology is a strong predictor of the rate of df and host (accuracy: 0.74‚ and 0.81, respectively). the best sperm morphology cut off point for df and host rate prediction was 4.5% and 5.5%, respectively.sperm morphology had significant correlation with df rate and host and is supposed to be a predictor for these tests. performing these three tests collectively for evaluation of semen samples would not be necessarily required in all cases.
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Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together?
Semen analysis, sperm DNA fragmentation (SDF) and hypo-osmotic swelling test (HOST) are usually performed for the evaluation of sperm fertilizing ability. There are some debates over the necessity of SDF and HOST incorporation in male infertility work-up.Semen of 77 men was evaluated by SDF and HOST through three semen analyses. Sperm parameters were arranged into different categories: <5%, 5-1...
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Semen analysis, sperm DNA fragmentation (SDF) and hypo-osmotic swelling test (HOST) are usually performed for the evaluation of sperm fertilizing ability. There are some debates over the necessity of SDF and HOST incorporation in male infertility work-up.Semen of 77 men was evaluated by SDF and HOST through three semen analyses. Sperm parameters were arranged into different categories: <5%, 5-1...
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Journal title:
international journal of molecular and cellular medicineجلد ۵، شماره ۴، صفحات ۰-۰
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